Mutations and polymorphisms in mitochondrial genome of dogs with solid mammary carcinoma – a preliminary study

In recent years, the association between mitochondrial DNA (mtDNA) changes and tumorigenesis has been discussed. contrast to human medicine, there are still few studies on molecular basis of canine tumors. One most commonly diagnosed, solid mammary carcinoma, is characterized by its aggressiveness, difficult treatment, generally poor prognosis. The aim study was reveal mutations polymorphisms in mtDNA dogs with carcinoma determine relationship these process neoplastic transformation. Blood, healthy tissue, tissue samples were collected from two crossbreed diagnosed G3 G2 carcinoma. Subsequently, for first time, Next Generation Sequencing (NGS) used analyze whole genome Thus, bioinformatic analyses included all 37 genes. As a result, 10 20 identified. Most polymorphisms/mutations found dog more advanced stage disease (G3). Twelve thirty identified have not described literature so far. These include eleven COX2 (m.7308A>G), ATP6 (m.8536C>T), ND4L (ins.9913_9914AG, ins.9913_9914TG, m.10165C>T), ND4 (m.10204C>T), CYTB (m.16248A>G, m.16268A>G), D-loop (m.16378G>A, m.16408G>A, m.16507T>A) which may be related tumorigenesis.